Post-translational modifications (PTMs) in splicing regulatory proteins and their effect on splicing regulation. Initially we will focus on PTMs in the splicing regulatory protein family: polypyrimidine tract binding protein (PTB) and homolog neuronal PTB (nPTB). Subsequently we will extend our studies to other splicing regulatory proteins such as the RNA binding Fox (RbFox) family of proteins. Our studies will utilize both in vivo and in vitro assays and related tissue culture and biochemical techniques.