The role of channels in the mitochondrial outer membrane in metabolic regulation and in the initiation of apoptosis:
highly interdisciplinary and includes growing and harvesting cells, isolating and purifying cell organelles, purifying soluble and membrane proteins, reconstituting proteins into vesicles and planar phospholipid membranes, making electrophysiological recordings on individual channel-forming molecules as well as ensembles of molecules, electron-microscopic studies of channel-forming proteins both by negative staining and freeze-fracture, and studies of genetically-modified channels.
