Job ID: 126966

Research Technician II

University at Buffalo, The State University of New York

  • Aug. 1, 2019
  • Research Technician II
  • Medicine
  • University at Buffalo, The State University of New York
    Buffalo, NY
  • Open until filled
  • Available immediately
  • Research Scientist/Associate
  • Biomedical Sciences
    Biology - General
    Sciences - General

Position Summary
The research lab of John Crane, MD, PhD, is actively recruiting for a Research Technician II.

  • Working safely with pathogenic bacteria and/or antibiotic-resistant bacteria under Biosafety Level-2 conditions. Prepare bacterial inocula for animal infection experiments and in vitro experiments. Ensure disposal of regulated medical waste using proper procedures.
  • Prepare antibiotic-containing microbiology media for detection and quantitation of emergence of antibiotic resistance in vitro and in vivo.
  • Assist in rabbit surgery experiments as surgical assistant in animal Operating Room using sterile technique.
  • Analyze intestinal fluid from rabbits for markers of inflammation such as myeloperoxidase (MPO) and other markers.
  • Perform a variety of other biochemical assays such as uric acid assays, Bio-Rad protein assays, Shiga toxin assays, etc. using the 96 well spectrophotometric plate reader
  • Perform real-time quantitative polymerase chain reaction (qRT-PCR) experiments to analyze bacteria for acquisition of antibiotic resistance genes.
  • Perform gel shift experiments to analyze binding of proteins of interest, such as RecA, to DNA .
  • Help supervise students doing rotations in the laboratory.
  • Keep track of amounts of chemicals and supplies on hand; Order or re-order chemicals and supplies when needed using the UB eReq system.
  • We may resume growing mammalian cells under sterile conditions and use for experiments such as adherence assays, cell death assays, ATP release, etc.
  • Photograph mammalian cells and bacteria using microscope and digital camera
  • Analyze data from experiments using Microsoft Excel; graph data using GraphPad Prism graphing software
  • Keep track of amounts of chemicals and supplies on hand; Order or re-order chemicals and supplies when needed using the UB eReq system.
  • Communicate scientific results with colleagues including collaborators
  • Maintain accurate records of experiments in the form of a laboratory notebook.
  • Wash laboratory dishes and glassware using the automatic glassware washer.
  • Perform other laboratory chores such as autoclaving, sanitizing the water system, changing CO2 tanks for CO2 incubator, autoclaving and disposal of biomedical waste, etc.

The Research Foundation is an AA/EOE employer and welcomes all to apply including persons with disabilities and veterans.

The University at Buffalo is SUNY’s most comprehensive public research university, and an outstanding place to work. UB amplifies ambition for faculty and staff by offering endless possibilities to achieve more. Here, people from all backgrounds and cultures challenge and inspire each other to discover, learn and succeed. Dedicated staff and engaged faculty collaborate to further knowledge and understanding, and develop tenacious graduates who are valued for their talents and their impact on global society. Visit our website to learn more about the University at Buffalo.

Minimum Qualifications
Bachelor’s degree required, preferably in a science-related major with 1 year of laboratory experience.

Preferred Qualifications
Master’s Degree

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