Staff Research Associate I (9613C) - 27480
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Application Review Date
The First Review Date for this job is: August 20, 2019
The Nayak lab studies the physiology and evolution of methanogenic archaea. The SRA will assist with performing routine molecular biology experiments towards the lab's goals of generating randomly barcoded transposon mutant libraries. Primary duties of the research technician will include performing standard molecular biology techniques such as PCR for DNA amplification, gel electrophoresis and Gibson assembly reactions. Other duties will include routine microbiology cultivation techniques and maintenance of lab equipment, reporting results and assisting in drafting manuscripts. Ideal candidates would have prior experience in working with microorganisms and performing transposon mutagenesis.
Molecular biology - Routine experiments including but not limited to:
- Designing primers to perform PCR amplification of DNA
- Gel electrophoresis
- Plasmid DNA extraction and DNA purification
- DNA ligation using the Gibson assembly technology
- Sample preparation for sequencing
- Analysis of sequencing data.
Cultivation of Microorganisms - Routine experiments including but not limited to:
- Preparation of growth media and other reagents
- Using the spectrophotometer to measure optical density and assay growth rates
- Setting up liposome mediated transformation assays with Methanosarcina
- Maintaining the anaerobic growth chambers
- B.S. in microbial biology and at least one year of experience working at the laboratory bench and/or equivalent experience/training.
- The candidate should be familiar with routine lab instruments such as a thermocycler, measuring balance, spectrophotometer, and a microcentrifuge.
- Familiarity with molecular biology techniques such as PCR, DNA extraction, plasmid purification and gel electrophoresis.
- Familiarity with sterile techniques for the cultivation of microorganisms and follow procedures to prepare growth media, reagents and buffers.
- Prior experience cultivating microorganisms and performing transposon mutagenesis
is strongly preferred.
Salary & Benefits
This position is a non-exempt, bi-weekly paid position. Hourly pay for this role is commensurate with experience within the range of $21.36 - $23.27, Steps 1.0 - 5.0.
For information on the comprehensive benefits package offered by the University visit:
How to Apply
Please submit your cover letter and resume as a single attachment when applying.
This position is governed by the terms and conditions in the agreement for the Research Support Professionals Unit (RX) between the University of California and the University Professional and Technical Employees (UPTE). The current bargaining agreement manual can be found at: http://ucnet.universityofcalifornia.edu/labor/bargaining-units/rx/index.html
Conviction History Background
This is a designated position requiring fingerprinting and a background check due to the nature of the job responsibilities. Berkeley does hire people with conviction histories and reviews information received in the context of the job responsibilities. The University reserves the right to make employment contingent upon successful completion of the background check.
Equal Employment Opportunity
The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or protected veteran status. For more information about your rights as an applicant see: http://www.eeoc.gov/employers/upload/poster_screen_reader_optimized.pdf
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