A long-standing, productive lab in the general area of the Cell Biology of Metals has been awarded a new, 5-year grant from the National Institutes of Neurological Diseases and Stroke as part of this institute’s focus on neurodegenerative diseases. The title of this grant is "Ferroportin and APP: Regulation of Iron Trafficking at the Blood-Brain Barrier." The premise of our work is that a key physiologic role of the amyloid precursor protein is to modulate the efflux of iron into the brain at the blood-brain barrier. Highlighted in this research is the use of a strongly validated BBB model based on the transwell cell system employing human and rodent brain microvascular capillary endothelial cells. Included in this research are studies on the regulation of this iron trafficking by astrocytes, and the uptake and efflux of iron by primary hippocampal neurons. Thus, our objective is to delineate the pattern of iron distribution in the neurovascular unit and its modulation by APP.
The work in the lab is highly collaborative and collegial. The research requires knowledge if not expertise in protein expression, purification and characterization; biophysical techniques such as circular dichroism and isothermal calorimetry; mammalian cell culture; plasmid and viral delivery of DNA for heterologous gene expression, RNAi and CRISP/Cas; bright field and fluorescence microscopy including indirect immunofluorescence and FRET. Clearly, the position includes a very strong training component, positioning the successful incumbent competitively for subsequent professional development.
The successful candidate will have the special treat of occupying the brand-new Jacobs School of Medicine and Biomedical Sciences building on the Buffalo-Niagara Medical Campus adjacent to the lively, expanding "millennial" center of Buffalo (http://www.buffalo.edu/here.html).
1. Ph.D. in Biochemistry/Molecular Biology/Cell Biology/Analytical Chemistry
2. Peer-reviewed publications in quality journals appropriate to field, preferably first-author
3. Strong written and oral communication skills including participation in research seminars/meetings/workshops (posters, presentations)
4. Demonstrated ability to work collaboratively
5. Experience in supervision/project responsibility role.
This candidate will have demonstrated scientific productivity and clear scientific communication, both written and oral. A strong command of English is essential
Eligibility for extramural fellowship support preferred.
Proficiency in the following techniques:
Contemporary recombinant DNA approaches
Cell culture, particularly mammalian cells
Protein Liquid Chromatography (protein purification)
Enzyme kinetic and metabolite assays
Protein biophysical techniques (spectrophotometry, isothermal calorimetry, CD, EPR)
Contact’s Name Daniel Kosman, PhD.
Contact’s Title UB Distinguished Professor, Biochemistry
Contact’s Email firstname.lastname@example.org
Contact’s Phone 716-829-2842